Fluorescence Microscopy - Theory and Practice ( Micaela Grandolfo )

The "fluorescence microscopy" course starts from the explanation of the phenomenon of fluorescence to arrive at how it is used in microscopy. The student is explained how a fluorescence microscope works, what its components are, how they are made and how to read the characteristics of the different types of filters. The features of the fluorophores are also studied in depth to allow the student to choose the most suitable one for his/her experiment, to combine them  correctly  in a multiple staining and, above all, to avoid low-performing fluorophores very present on the market. The second part of this course is entirely dedicated to confocal microscopy and all the peculiarities of this technique are deepened: from the type of light source, to the use of the pinhole, to the functioning of PMTs and sampling in z; deepening how much the regulation of these parameters affect the quality of the final image After the lectures are planned practice sessions.